Multi-isotopic and morphometric evidence for the migration of farmers leading up to the Inka conquest of the southern Andes.

We present isotopic and morphometric evidence suggesting the migration of farmers in the southern Andes in the period AD 1270-1420, leading up to the Inka conquest occurring ~ AD 1400. This is based on the interdisciplinary study of human remains from archaeological cemeteries in the Andean Uspallata Valley (Argentina), located in the southern frontier of the Inka Empire. The studied samples span AD 800-1500, encompassing the highly dynamic Late Intermediate Period and culminating with the imperial expansion. Our research combines a macro-regional study of human paleomobility and migration based on a new strontium isoscape across the Andes that allows identifying locals and migrants, a geometric morphometric analysis of cranio-facial morphology suggesting separate ancestral lineages, and a paleodietary reconstruction based on stable isotopes showing that the migrants had diets exceptionally high in C4 plants and largely based on maize agriculture. Significantly, this migration influx occurred during a period of regional demographic increase and would have been part of a widespread period of change in settlement patterns and population movements that preceded the Inka expansion. These processes increased local social diversity and may have been subsequently utilized by the Inka to channel interaction with the local societies.

Distribution of maternal lineages in hunter-gatherer societies of the southern coast of Tierra del Fuego, Argentina.

OBJECTIVES: The aim of this work is to explore the maternal genetic diversity of hunter-gatherers of the southern Tierra del Fuego, specifically the north coast of Beagle Channel, the Península Mitre, and Isla de los Estados through ancient mitochondrial DNA analysis. MATERIALS AND METHODS: The hypervariable regions 1 and 2 of the mitochondrial genome of five individuals from the north coast of Beagle Channel, six individuals from Península Mitre, and one individual from Isla de los Estados were analyzed. Through diversity statistics, Analysis of Molecular Variance (AMOVA), and Median Joining networks analyses, maternal relationships in the region were evaluated and phylogenetic similarities between ancient and contemporary populations of Tierra del Fuego were determined. RESULTS: The mitochondrial DNA lineages from the ancient individuals analyzed reveals the presence of subclades C1b and D1g. Pattern of decreasing genetic diversity toward the South is observed. The AMOVAs performed found no statistically significant differences between individuals of the north coast of Beagle Channel and Península Mitre-Isla de los Estados, and modern Yámana populations. Median joining network of haplotypes of clades C1 and D1g, show the same results. DISCUSSION: Ethnohistoric and ethnographic records of Península Mitre show that this region was occupied during the 19th century by Haush or Manekenk populations, although their biological, cultural, and subsistence characterization is unclear. We explore their maternal lineages and encounter low levels of genetic diversity and the absence of population differentiation with modern Yámana groups. We suggest that Península Mitre-Isla de los Estado was part of the same hunting and gathering populations as those of the Beagle Channel.

Scale of human mobility in the southern Andes (Argentina and Chile): A new framework based on strontium isotopes.

OBJECTIVES: The goal of this article is to assess the scale of human paleomobility and ecological complementarity between the lowlands and highlands in the southern Andes during the last 2,300 years. By providing isotope results for human bone and teeth samples, we assess a hypothesis of "high residential mobility" suggested on the basis of oxygen isotopes from human remains. METHODS: We develop an isotopic assessment of human mobility in a mountain landscape combining strontium and oxygen isotopes. We analyze bone and teeth samples as an approach to life-history changes in spatial residence. Human samples from the main geological units and periods within the last two millennia are selected. RESULTS: We present a framework for the analysis of bioavailable strontium based on the combination of the geological data with isotope results for rodent samples. The 87 Sr/86 Sr values from human samples indicate residential stability within geological regions along life history. When comparing strontium and oxygen values for the same human samples, we record a divergent pattern: while δ18 O values for samples from distant regions overlap widely, there are important differences in 87 Sr/86 Sr values. CONCLUSIONS: Despite the large socio-economic changes recorded, 87 Sr/86 Sr values indicate a persisting scenario of low systematic mobility between the different geological regions. Our results suggest that strontium isotope values provide the most germane means to track patterns of human occupation of distinct regions in complex geological landscapes, offering a much higher spatial resolution than oxygen isotopes in the southern Andes.

Dietary resilience among hunter-gatherers of Tierra del Fuego: Isotopic evidence in a diachronic perspective.

The native groups of Patagonia have relied on a hunter-gatherer economy well after the first Europeans and North Americans reached this part of the world. The large exploitation of marine mammals (i.e., seals) by such allochthonous groups has had a strong impact on the local ecology in a way that might have forced the natives to adjust their subsistence strategies. Similarly, the introduction of new foods might have changed local diet. These are the premises of our isotopic-based analysis. There is a large set of paleonutritional investigations through isotopic analysis on Fuegians groups, however a systematic exploration of food practices across time in relation to possible pre- and post-contact changes is still lacking. In this paper we investigate dietary variation in hunter-gatherer groups of Tierra del Fuego in a diachronic perspective, through measuring the isotopic ratio of carbon (∂13C) and nitrogen (∂15N) in the bone collagen of human and a selection of terrestrial and marine animal samples. The data obtained reveal an unexpected isotopic uniformity across prehistoric and recent groups, with little variation in both carbon and nitrogen mean values, which we interpret as the possible evidence of resilience among these groups and persistence of subsistence strategies, allowing inferences on the dramatic contraction (and extinction) of Fuegian populations.

Isotopic evidence of weaning in hunter-gatherers from the late holocene in Lake Salitroso, Patagonia, Argentina.

OBJECTIVES: The timing and duration of breastfeeding and weaning in past hunter-gatherer populations are discussed based on the results of stable carbon and nitrogen isotope analyses undertaken on a Late Holocene skeletal sample from Lake Salitroso in the Patagonian steppe (Argentina). Research in Lake Salitroso is part of a regional project that studies the relationship between hunter-gatherer societies, their organizational systems and the environmental changes during the last 3,000 years in Patagonia. METHODS: The sample included 52 individuals: 33 subadults and 19 adults of both sexes. They were recovered from 24 burial stone structures, locally called chenques, with dates ranging from ca. 800 BP to 350 BP. Ribs were selected for collagen extraction and measurement of (13) C/(12) C and (15) N/(14) N ratios. RESULTS: A δ(13) C mean value of -18.7‰ ± 0.5‰ was obtained, with a range between -19.8‰ and -17.1‰ whereas δ(15) N recorded a mean of 11.9‰ ± 1.1‰ with a range between 10.1‰ and 14.8‰. δ(15) N data showed an age-related pattern with particularly marked difference between values for subadults under the age of 4 and older individuals. As opposed to δ(15) N, δ(13) C showed little variation with age. CONCLUSIONS: An early incorporation of supplementary solid food between 0.75 and 2 years of age and a late cessation of breastfeeding at about 5-6 years of age were inferred. This suggests that among this Patagonian hunter-gatherer population weaning was a gradual and lengthy process. These results are consistent with the patterns observed in cross-cultural studies and archaeological samples of hunter-gatherer groups.